| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073567 |
| Chromosome: | chromosome 2 |
| Location: | 1378642 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083259 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAACGCCCAGATGATGAGCAGTATGC |
| Internal bar code: | TTATAACAACTGGCGCGAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 97.754 |
| LEAP-Seq n confirming: | 914 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGATCAGACCCAGCAG |
| Suggested primer 2: | TTTAAACGCCCCAGCATTAG |