| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073641 |
| Chromosome: | chromosome 7 |
| Location: | 4614817 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344186 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCAAACACGGCGCTATGGGCAGCTGT |
| Internal bar code: | GTCTACGCATGGCGATTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 98.9418 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGATTGAGTTTGTGGGTC |
| Suggested primer 2: | GCTACGCCTGGTCAAAGAAG |