Insertion junction: LMJ.RY0402.073668_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073668
Chromosome:chromosome 4
Location:2770957
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g223750 (1 of 1) IPR000048//IPR009071//IPR027417 - IQ motif, EF-hand binding site // High mobility group box domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGATGTGAGGGTGACGGTGACGCTGAGTT
Internal bar code:CCGCGTGACTGAATTGAGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:240
LEAP-Seq percent confirming:57.5825
LEAP-Seq n confirming:4135
LEAP-Seq n nonconfirming:3046
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTGGCAACATCAACAGC
Suggested primer 2:AGGGTGTGTTCTTCCGTCAC