| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073697 |
| Chromosome: | chromosome_6 |
| Location: | 6144607 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g290200 | CYG39 | Adenylate/guanylate cyclase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCCAGGTCCACCTGACGTCCACGCCAGC |
| Internal bar code: | CAGTCCACAACGAGGTGCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTTTGGAAAAGCGGTA |
| Suggested primer 2: | CGTTATGAACGCGAGTGCT |