| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073738 |
| Chromosome: | chromosome 1 |
| Location: | 7959393 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g067282 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCATGCTCATGCATACCACCTTGTAACC |
| Internal bar code: | GGTTATCATACTAGAGTAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 99.7668 |
| LEAP-Seq n confirming: | 5133 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGACCCAGGTTGCTAAGT |
| Suggested primer 2: | ATTTTGTCGCACGGGATAAG |