| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073746 |
| Chromosome: | chromosome 16 |
| Location: | 6132361 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675650 | ALDH6,ALD8,ALDH6B1 | (1 of 1) 1.2.1.27 - Methylmalonate-semialdehyde dehydrogenase (CoA acylating) / MSDH; Aldehyde dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGAATGCTCGGAAGGGCGGGTGGGCG |
| Internal bar code: | CAGCGTGTTACTTCAAGCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 69.1218 |
| LEAP-Seq n confirming: | 244 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACTACGCCCTTGACGAG |
| Suggested primer 2: | GGTACCGTAATGCACAGCCT |