Insertion junction: LMJ.RY0402.073754_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073754
Chromosome:chromosome 2
Location:5306332
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g106750 uS9m,MRPS9 (1 of 2) PTHR21569//PTHR21569:SF1 - RIBOSOMAL PROTEIN S9 // 28S RIBOSOMAL PROTEIN S9, MITOCHONDRIAL; Mitochondrial ribosomal protein S9 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCATGTTCCGCCTGGACCGCACGCAGCC
Internal bar code:GCACCTAGTAAGCCCGTAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:512
LEAP-Seq percent confirming:97.5047
LEAP-Seq n confirming:3087
LEAP-Seq n nonconfirming:79
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACCATGAATCGCTCGTATG
Suggested primer 2:CTGTCGTCGTACTTCCGTGA