| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073757 |
| Chromosome: | chromosome 16 |
| Location: | 4286289 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668050 | (1 of 97) PF13650 - Aspartyl protease (Asp_protease_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCTGCCCTTCCAACCCTCCAACCATGG |
| Internal bar code: | GCATGGTGGGAGCTGAGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 58 |
| LEAP-Seq percent confirming: | 89.9373 |
| LEAP-Seq n confirming: | 4737 |
| LEAP-Seq n nonconfirming: | 530 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACTCATACTCGCCGCCT |
| Suggested primer 2: | ACTGTTCAGGTGGTCAAGGG |