| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073775 |
| Chromosome: | chromosome 4 |
| Location: | 478048 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217929 | (1 of 1) 1.8.2.1//1.8.3.1 - Sulfite dehydrogenase // Sulfite oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGTTTGGGCTGGTACTTACACCCCCTC |
| Internal bar code: | ATCCAGGATCACCGGGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 85.6604 |
| LEAP-Seq n confirming: | 227 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTCGCAACTCATTGAA |
| Suggested primer 2: | CCCCTCTGTCTACTTCGCTG |