| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073777 |
| Chromosome: | chromosome 3 |
| Location: | 4042742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172322 | (1 of 3) PTHR15439//PTHR15439:SF4 - RETINOBLASTOMA-BINDING PROTEIN 6 // ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 24 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGAAGTTAGGTCTTCGAATCAATGAA |
| Internal bar code: | TCTTGTCTGTCAAATTAACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.5599 |
| LEAP-Seq n confirming: | 7466 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTCCTGTTCCCTTCTTT |
| Suggested primer 2: | CCTCACTCCTTGCCTCACTC |