| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073795 |
| Chromosome: | chromosome 2 |
| Location: | 1830200 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACCACATGCCAGCGCTGCCCAGCCG |
| Internal bar code: | TGAATTCTAGAATCCCTCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 96.7001 |
| LEAP-Seq n confirming: | 674 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTCTAAGCCGCATCAA |
| Suggested primer 2: | GGTTATCACTCGTGACGCCT |