| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073809 |
| Chromosome: | chromosome 3 |
| Location: | 3160913 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGCATGTGTGTGTTAAAAAACGAAA |
| Internal bar code: | AATGAAAACGTTCGGGCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 33.1658 |
| LEAP-Seq n confirming: | 3828 |
| LEAP-Seq n nonconfirming: | 7714 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCCAGCACGCCTAATTC |
| Suggested primer 2: | CCCAGTATACACCCACCCAC |