| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.073834 |
| Chromosome: | chromosome 2 |
| Location: | 8752656 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142366 | (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGCCTCAGCCCGTCTCAGCCTCAGGC |
| Internal bar code: | CTTGTCGGCCATTGTTGAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 22.8739 |
| LEAP-Seq n confirming: | 234 |
| LEAP-Seq n nonconfirming: | 789 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGAAGCAGGGCGATGAG |
| Suggested primer 2: | TGTGCCTGTCAAGACCTCAG |