| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073842 |
| Chromosome: | chromosome 2 |
| Location: | 3721141 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGATAGCGAAGGGCTTCTGCGCGCCTGG |
| Internal bar code: | ACGCCTAGATGCAGATAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.7067 |
| LEAP-Seq n confirming: | 680 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGCCAGGTCCAGTAGC |
| Suggested primer 2: | GTCAGCCAGCTGTTCCTTTC |