| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073860 |
| Chromosome: | chromosome 3 |
| Location: | 8343749 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198850 | TL19 | (1 of 3) PTHR31407//PTHR31407:SF18 - FAMILY NOT NAMED // PSBP DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC; Thylakoid llumen protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAGCCTCGGCCTCGCGCTCGCAGCGGG |
| Internal bar code: | AGAGCTTGTGGCGGGAAAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 52.8112 |
| LEAP-Seq n confirming: | 526 |
| LEAP-Seq n nonconfirming: | 470 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATTACAGCGGGTGGTA |
| Suggested primer 2: | GGAAACTCGGCAGCTACAAG |