| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073869 |
| Chromosome: | chromosome 17 |
| Location: | 848416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702351 | HLM26 | putative N-methyltransferase; (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCGGCCTCAAAATCAACGTTATCCGGA |
| Internal bar code: | GGATCTCGTACCTGGTACCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 95.2663 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTTGTGGGCCGTAAAAG |
| Suggested primer 2: | CTAAGAAACGGGCAGAAACG |