| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.073873 |
| Chromosome: | chromosome 10 |
| Location: | 6518522 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466750 | SAT1 | (1 of 1) PTHR23416//PTHR23416:SF2 - SIALIC ACID SYNTHASE-RELATED // SERINE ACETYLTRANSFERASE 5; Serine O-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGTCCTGGTGCTCGTCCTAGCCGCTAC |
| Internal bar code: | TCATCGCCGCACGTATAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.6413 |
| LEAP-Seq n confirming: | 1111 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCATCATCTGATCTCTCA |
| Suggested primer 2: | GGTTGACGGGTGTCTTGAGT |