| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073895 |
| Chromosome: | chromosome 14 |
| Location: | 3472857 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630787 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCTGGTATGGACCGGGGTTGCTGTGTT |
| Internal bar code: | GGGGAGCACAGGGGAAGAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 34.5567 |
| LEAP-Seq n confirming: | 1403 |
| LEAP-Seq n nonconfirming: | 2657 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACGCTCGCGCACTACTT |
| Suggested primer 2: | AGTTAGGTTGATGATGGCGG |