| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073912 |
| Chromosome: | chromosome 14 |
| Location: | 3036377 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628237 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGGCAGACATGGCCGCAGAGTCCTCA |
| Internal bar code: | GCCGGTGATAGGCGTACCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 50.7932 |
| LEAP-Seq n confirming: | 1665 |
| LEAP-Seq n nonconfirming: | 1613 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCACTGTCGTACATGCC |
| Suggested primer 2: | CAACTGTAAGCGCACTCTGC |