Insertion junction: LMJ.RY0402.073929_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.073929
Chromosome:chromosome 3
Location:1974205
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g155750 PHC65 Putative pherophorin-chlamydomonas homolog; (1 of 1) IPR001304//IPR016186//IPR016187//IPR018081//IPR024616 - C-type lectin // C-type lectin-like // C-type lectin fold // Anaphylatoxin, complement system // Pherophorin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCCCCTCCTCCTAGCCCCAAGCCGCCC
Internal bar code:CCGCGGCTTCGCTGAATTACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:24.5614
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:129
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACAGGAGCTGGGGTTG
Suggested primer 2:GATAGGGGTGTCGTGCTGTT