| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.073957 |
| Chromosome: | chromosome 8 |
| Location: | 96804 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358529 | Similar to Flagellar Associated Protein FAP157 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGAAGCAACAATGCCGCACCCGCACCCT |
| Internal bar code: | CTCTCGTGCGGCACTTCAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 89.4587 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTCTTCCCTTTCCCTT |
| Suggested primer 2: | GTGGATGCATCAGTGTCCAG |