| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073983 |
| Chromosome: | chromosome 5 |
| Location: | 418395 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232750 | CNK4 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF311 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGACGGAGCATACCGGACTCGCTCGCA |
| Internal bar code: | TCGCGAATTCCGTCGAGACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTGGATGCTGCAGTTA |
| Suggested primer 2: | ACAAGTCCGAGGTCATCCAG |