| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.073993 |
| Chromosome: | chromosome 7 |
| Location: | 5062712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347550 | (1 of 1) PTHR23389:SF6 - ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCGCAATGGCGGCAGTGGATGCGGCG |
| Internal bar code: | GGCCGGAGGCGAGAACCATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 64.2126 |
| LEAP-Seq n confirming: | 1317 |
| LEAP-Seq n nonconfirming: | 734 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAGACATGTAGTCCGGG |
| Suggested primer 2: | CTCGTCCTCCTCATCGAGAC |