| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074006 |
| Chromosome: | chromosome 1 |
| Location: | 6004898 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042550 | (1 of 1) PTHR21573:SF0 - ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGACCGGGGGCCCAAGTGTAAGTTCA |
| Internal bar code: | AAACCGAGCGCCCTCAAGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.2905 |
| LEAP-Seq n confirming: | 26591 |
| LEAP-Seq n nonconfirming: | 190 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGGTCATAACGCATTG |
| Suggested primer 2: | CCCAAGAAGCAAAAGCTGAC |