Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.074039 |
Chromosome: | chromosome 14 |
Location: | 1063865 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g614750 | CPNAT1,cpNAT1,NAT34 | (1 of 1) 2.3.1.189 - Mycothiol synthase; Ribosome-associated chloroplast-localized N-acetyltransferase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGAGATGCTGTGAAGCCGCGCTGCGC |
Internal bar code: | CACTCAGAACGTCACCTTCCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 554 |
LEAP-Seq percent confirming: | 99.5526 |
LEAP-Seq n confirming: | 445 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAGTTCAAGTCGGCTTTCG |
Suggested primer 2: | CTTCGTGCCTCTGTGTTTGA |