Insertion junction: LMJ.RY0402.074039_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.074039
Chromosome:chromosome 14
Location:1063865
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g614750 CPNAT1,cpNAT1,NAT34 (1 of 1) 2.3.1.189 - Mycothiol synthase; Ribosome-associated chloroplast-localized N-acetyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGGGAGATGCTGTGAAGCCGCGCTGCGC
Internal bar code:CACTCAGAACGTCACCTTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:99.5526
LEAP-Seq n confirming:445
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTTCAAGTCGGCTTTCG
Suggested primer 2:CTTCGTGCCTCTGTGTTTGA