Insertion junction: LMJ.RY0402.074061_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.074061
Chromosome:chromosome 11
Location:845073
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467649 OPR107 OctotricoPeptide Repeat protein 107; (1 of 1) IPR010622//IPR016024 - FAST kinase leucine-rich // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCGTGTGCTCACATCCGCTCCCACACT
Internal bar code:TCCGTGAGGGGGGTTCCATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:343
LEAP-Seq percent confirming:99.6681
LEAP-Seq n confirming:901
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCGCAGCAATCAGTATG
Suggested primer 2:ACTCCGAACTGGAAGAGCAA