| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074077 |
| Chromosome: | chromosome 9 |
| Location: | 5195693 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399289 | (1 of 3) PF00651//PF03110 - BTB/POZ domain (BTB) // SBP domain (SBP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCGGAGCTTCTCTCACGGCTTCACATC |
| Internal bar code: | GGTCTTACTCGTGCTTGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.3507 |
| LEAP-Seq n confirming: | 7345 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTCTCCCTGGTCCTTTCA |
| Suggested primer 2: | TGGGCTGGGTTGCTATTATC |