| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074089 |
| Chromosome: | chromosome 9 |
| Location: | 6794019 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409500 | RPP25 | Ribonuclease MRP subunit P25; (1 of 2) PF01918 - Alba (Alba) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTTGTGCGTGCAGCGGGCAGTCGTGAC |
| Internal bar code: | ACAACTCTGTACGCTCGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 94.8183 |
| LEAP-Seq n confirming: | 11821 |
| LEAP-Seq n nonconfirming: | 646 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCGGCCTCTAATTCTG |
| Suggested primer 2: | AAGGCAAAGAAAGGCTGTGA |