| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074102 |
| Chromosome: | chromosome 6 |
| Location: | 8016488 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304050 | PKC1 | Protein Kinase C; (1 of 3) K15710 - E3 ubiquitin-protein ligase SHPRH [EC:3.6.4.- 6.3.2.19] (SHPRH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGCCTGCAGGCTGAGAATGTGGTGTT |
| Internal bar code: | CGTATGGAGGCGTTGATGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 90.9658 |
| LEAP-Seq n confirming: | 8609 |
| LEAP-Seq n nonconfirming: | 855 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTAGGGCTGAAGTGCTTG |
| Suggested primer 2: | GCCGTACATGTCAGGATGTG |