| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074115 |
| Chromosome: | chromosome 12 |
| Location: | 1049604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491050 | RIR2A,DIV17,NSG2,RIR2 | (1 of 2) K10808 - ribonucleoside-diphosphate reductase subunit M2 (RRM2); Ribonucleoside-diphosphate reductase R2 subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCGTAAGCCAACATGCCTCAAAGACG |
| Internal bar code: | GACTTGCAAGGAAACGGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACCAATCCTAGGCACAA |
| Suggested primer 2: | TCAAGCAGATCATCGTGGAG |