| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074119 |
| Chromosome: | chromosome 10 |
| Location: | 6316846 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465300 | (1 of 89) 3.6.4.13 - RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCAGTCTTGCGTCAGCACAGCAATCGG |
| Internal bar code: | CGGGCGGTTCGGGAGCGTCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 58 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCCCTCCCCTTTCTGAC |
| Suggested primer 2: | TGTCTGTGCGCTTTGTTTTC |