| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074121 |
| Chromosome: | chromosome_12 |
| Location: | 7089719 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g559400 | PDE12 | 3\',5\'-cyclic-nucleotide phosphodiesterase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGCCTTCCTGCCGCCGCCGCCCCCGCCAC |
| Internal bar code: | GGGCAGAATTATCGGTAATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTCACGTGGAGTTAGTT |
| Suggested primer 2: | GACCACAGTGAAAGCAGCAA |