Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.074128 |
Chromosome: | chromosome 15 |
Location: | 505487 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATTGGGGGTGCAAGAGCGACTCCGGAG |
Internal bar code: | TCCCCAGTTATCTCGCGCGGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 200 |
LEAP-Seq percent confirming: | 95.0437 |
LEAP-Seq n confirming: | 2608 |
LEAP-Seq n nonconfirming: | 136 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTGGTAGTCGGGTGTGTG |
Suggested primer 2: | GCTAGCGCTATTGCTCTGCT |