| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074138 |
| Chromosome: | chromosome 12 |
| Location: | 2635463 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504400 | (1 of 1) 2.3.1.22 - 2-acylglycerol O-acyltransferase / Monoglyceride acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCCGCAGCCTCCTCAATAAGGCTCTT |
| Internal bar code: | AGCCAAGTCGTTTCGCTTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 78.7879 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCTGATCTATGATGCT |
| Suggested primer 2: | ACCGTAATGGCAATCGACTC |