| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.074142 |
| Chromosome: | chromosome 3 |
| Location: | 2556131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160300 | RAM1,RAMP4 | Ribosome-associated membrane protein, ER-stress-associated; (1 of 1) PF06624 - Ribosome associated membrane protein RAMP4 (RAMP4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATAGCCTCATACGGTACCGCGGCTGTA |
| Internal bar code: | CGATTCTGAGGTAATGTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 96.89 |
| LEAP-Seq n confirming: | 810 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGTGGCGACCAAATCT |
| Suggested primer 2: | TGTGCCCAAGATCAAAATCA |