| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074149 |
| Chromosome: | chromosome 13 |
| Location: | 1955209 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576500 | (1 of 1) PTHR35698//PTHR35698:SF1 - FAMILY NOT NAMED // DNA-BINDING PROTEIN RHL1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTTCCTCAGGCTCCTTTTTGGCCTTC |
| Internal bar code: | TGGACCGTAGGCTCCGCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGACTAGGGGAGGGGAG |
| Suggested primer 2: | GAAGGCTGTTAGCGACAAGG |