| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074157 |
| Chromosome: | chromosome 3 |
| Location: | 4051388 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172451 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCTGCACCAATTTTGTGAGGACAACAT |
| Internal bar code: | GGGGCCATGGGTATTGTGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 90.0482 |
| LEAP-Seq n confirming: | 1683 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCAGCCAAGACCAGGAG |
| Suggested primer 2: | GATCCTTGGCCATCGTCTTA |