| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074181 |
| Chromosome: | chromosome 12 |
| Location: | 8510119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547900 | MCP27 | Mitochondrial substrate carrier protein; (1 of 1) PTHR24089:SF242 - MITOCHONDRIAL COENZYME A TRANSPORTER SLC25A42 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGAGTGCCTCGTAGGTGGTGAAGGCAA |
| Internal bar code: | ACGCGAACCTCAACGTATAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 92.8943 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACAACTGCATCCTCAAG |
| Suggested primer 2: | CGAAACCTTTCCTGTACCCA |