| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074184 |
| Chromosome: | chromosome 16 |
| Location: | 5646624 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679400 | (1 of 1) PF16501 - S phase cyclin A-associated protein in the endoplasmic reticulum (SCAPER_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGCAAGCGTAAAGGCAGTTCAGGCAC |
| Internal bar code: | GTAGCTTTACGCCTTGCAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGACAAGAAGATGGC |
| Suggested primer 2: | TCTGTGGTGTTGTTGGGTTG |