| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074189 |
| Chromosome: | chromosome 5 |
| Location: | 1778718 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242300 | EIF3D | Eukaryotic translation initiation factor 3, subunit D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCACGGAGTCCGCAGAGCGCACAGCCC |
| Internal bar code: | TGCGACTTATGGACAGGACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 95.6222 |
| LEAP-Seq n confirming: | 2359 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGTATTCAGTCGAGCA |
| Suggested primer 2: | GCAGAACAATCGCTTCAACA |