| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074211 |
| Chromosome: | chromosome 13 |
| Location: | 4922548 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606050 | TSY1 | Tyrosyl-tRNA synthetase; (1 of 1) PTHR11766//PTHR11766:SF0 - TYROSYL-TRNA SYNTHETASE // TYROSINE--TRNA LIGASE, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTATCCAACAAATCCCCCGCCCGACTTC |
| Internal bar code: | ATTCGTGAAGGTTGTGCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 98.2143 |
| LEAP-Seq n confirming: | 220 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTCGTACTCCACGTTCG |
| Suggested primer 2: | GTTGTATGGTGCGTCTGGTG |