| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074222 |
| Chromosome: | chromosome 6 |
| Location: | 3914113 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278183 | (1 of 1) PTHR23054//PTHR23054:SF14 - UNCHARACTERIZED // PROTEIN Y45F10A.7, ISOFORM A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCATTCGGCTTACACGGCTGCGAGGACG |
| Internal bar code: | AAAGCGCGGGTGTTGATGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 81.0892 |
| LEAP-Seq n confirming: | 1355 |
| LEAP-Seq n nonconfirming: | 316 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGGAAGAACGACAACCC |
| Suggested primer 2: | GGCTTGGCTAGGACTCACTG |