| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074225 |
| Chromosome: | chromosome 8 |
| Location: | 1553963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365000 | CSE1 | Protein of unknown function; (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGAGGATGCGACGTTGCCCCGTGCTG |
| Internal bar code: | GCAAATCTCGTGCTCTTTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 80.0117 |
| LEAP-Seq n confirming: | 1369 |
| LEAP-Seq n nonconfirming: | 342 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCAGAGCACAAGGGAGG |
| Suggested primer 2: | CTTGAGGTGAGGAGGCAGAC |