| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.074243 |
| Chromosome: | chromosome 12 |
| Location: | 5499977 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531000 | AMT1H,AMT8 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCATGCGGAGATGGATGCTGGTTGTGTT |
| Internal bar code: | CGGCTCCCGATCCTGACGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 96.6116 |
| LEAP-Seq n confirming: | 2281 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCGATAACCAGACACCG |
| Suggested primer 2: | TGCTGGTGTTCTACCTGCTG |