Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.074251 |
Chromosome: | chromosome_16 |
Location: | 515991 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g692902 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGGTGAGTGGGGCCGCTCCTGCATACGC |
Internal bar code: | TTGGTCCCGCCTTATCAGGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 516 |
LEAP-Seq percent confirming: | 99.564 |
LEAP-Seq n confirming: | 1827 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATCCTCACTACACAGCGCA |
Suggested primer 2: | CACGCAATACAAAACCATGC |