| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074278 |
| Chromosome: | chromosome 2 |
| Location: | 4472625 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100250 | (1 of 1) K18211 - synaptosomal-associated protein 25 (SNAP25) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTATGTGTGTTAGCCTGTTAGGCAAGCAC |
| Internal bar code: | GAAGTCGGTGGGGGGTAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 85.3261 |
| LEAP-Seq n confirming: | 314 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTACCCAGGATTGTGCTG |
| Suggested primer 2: | ACGTGAACTCTCGGCACTCT |