| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.074308 |
| Chromosome: | chromosome 4 |
| Location: | 4059387 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g232902 | (1 of 77) IPR012340 - Nucleic acid-binding, OB-fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACTAAACCGAACCCAATGCAAGAGAG |
| Internal bar code: | AGTTTAGCGGCGCACGTCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 98.3296 |
| LEAP-Seq n confirming: | 883 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGTATCCACGCGTAGCC |
| Suggested primer 2: | CTCTTGCACGCTACCACAAA |