Insertion junction: LMJ.RY0402.074315_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.074315
Chromosome:chromosome 1
Location:190603
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g001300 ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACGCATGCACGCCACGCCACGCCACG
Internal bar code:AGGCGGGGGCGCGCTGGCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:130
LEAP-Seq percent confirming:15.8134
LEAP-Seq n confirming:1044
LEAP-Seq n nonconfirming:5558
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGTCCCAAAGGAAACAG
Suggested primer 2:TGCAGCAGATCATTGAGAGG