| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074321 |
| Chromosome: | chromosome 3 |
| Location: | 2433070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCTTGGCTGCTGGCTGCTGTATCCAGT |
| Internal bar code: | CCAAAGTACCTCAATAGGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.6795 |
| LEAP-Seq n confirming: | 2177 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTACCAAGAGGTTGCC |
| Suggested primer 2: | CACGGTCCGCAAATCTATCT |