| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.074331 |
| Chromosome: | chromosome_6 |
| Location: | 8519638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g308000 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCTTCTGCTCTACGTCTTCCCCTCCCCC |
| Internal bar code: | GAAGGTCAGATGTGTGGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 59.8522 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 163 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTACGGTGCACACACAT |
| Suggested primer 2: | CGAGCTCAAGTACCTACCGC |